Research Article

Study of gene expression of the ERK1/2 gene and its relationship to PGE2 levels in the serum of patients with Toxoplasma gondii

Abstract

Objective of this study, find the levels of PGE2 in serum for a group for male patients infected with Toxoplasma gondii and its relationship with the gene expression of the ERK gene. The patients were diagnosed with the disease by rapid IGg screening and to find the levels of IGg protein in the blood serum of a group of male patients infected with the disease. The aforementioned immune marker in the serum of healthy people as a control group. Blood samples were taken from donors flowing into the main blood bank in Najaf, and the relationship between this immune marker was studied using ELISA technology and its relationship to the gene expression of the ERK gene using real-time PCR. The total number of males was (78), including (48) patients and (30) healthy males who were not infected with the disease. The current results revealed that the serum levels of gene expression of ERK gene (pg/ml) correlated negatively and significantly with PGE (pg/ml) in patients infected with chronic Toxoplasmosis. The current study concluded that the disease is dangerous for males, and there are many complications in males when the disease worsens. It is dangerous to transfuse blood from donors without undergoing a Toxoplasma gondii examination, especially to people who have immunodeficiency.